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salmonella acanthamoeba co culture  (New England Biolabs)
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New England Biolabs salmonella acanthamoeba co culture
Gene set enrichment analysis performed using the fgsea package. Positive (normalized) enrichment scores indicate upregulation of genes in the pathway in Salmonella within <t>Acanthamoeba</t> . Negative (normalized) enrichment scores indicate downregulation of genes in the pathway in Salmonella within Acanthamoeba . Operon pathway annotation was determined against the KEGG database.
Salmonella Acanthamoeba Co Culture, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Gene set enrichment analysis performed using the fgsea package. Positive (normalized) enrichment scores indicate upregulation of genes in the pathway in Salmonella within Acanthamoeba . Negative (normalized) enrichment scores indicate downregulation of genes in the pathway in Salmonella within Acanthamoeba . Operon pathway annotation was determined against the KEGG database.

Journal: International Journal of Molecular Sciences

Article Title: Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence for Salmonella Typhimurium Intracellular Survival within Acanthamoeba castellanii

doi: 10.3390/ijms22169077

Figure Lengend Snippet: Gene set enrichment analysis performed using the fgsea package. Positive (normalized) enrichment scores indicate upregulation of genes in the pathway in Salmonella within Acanthamoeba . Negative (normalized) enrichment scores indicate downregulation of genes in the pathway in Salmonella within Acanthamoeba . Operon pathway annotation was determined against the KEGG database.

Article Snippet: The primers were designed using Vector NTI 9.1.0 (Thermo Fisher Scientific, Waltham, MA, USA) ( ). cDNA was synthesized from 1 µg RNA of Salmonella control culture or 3 µg RNA of Salmonella - Acanthamoeba co-culture using the M-MuLV Reverse Transcriptase (New England Biolabs, Ipswich, MA, USA).

Techniques:

Selected operons and genes that were differentially expressed in Salmonella within Acanthamoeba .

Journal: International Journal of Molecular Sciences

Article Title: Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence for Salmonella Typhimurium Intracellular Survival within Acanthamoeba castellanii

doi: 10.3390/ijms22169077

Figure Lengend Snippet: Selected operons and genes that were differentially expressed in Salmonella within Acanthamoeba .

Article Snippet: The primers were designed using Vector NTI 9.1.0 (Thermo Fisher Scientific, Waltham, MA, USA) ( ). cDNA was synthesized from 1 µg RNA of Salmonella control culture or 3 µg RNA of Salmonella - Acanthamoeba co-culture using the M-MuLV Reverse Transcriptase (New England Biolabs, Ipswich, MA, USA).

Techniques: Translocation Assay, Chemotaxis Assay

Acanthamoeba castellanii infected with S. Typhimurium (GFP) before ( A ) and after ( B ) washing procedures. Confocal laser scanning microscopy: differential interference contrast overlays with single optical GFP fluorescence sections in the green channel. Scale bars: 10 µm.

Journal: International Journal of Molecular Sciences

Article Title: Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence for Salmonella Typhimurium Intracellular Survival within Acanthamoeba castellanii

doi: 10.3390/ijms22169077

Figure Lengend Snippet: Acanthamoeba castellanii infected with S. Typhimurium (GFP) before ( A ) and after ( B ) washing procedures. Confocal laser scanning microscopy: differential interference contrast overlays with single optical GFP fluorescence sections in the green channel. Scale bars: 10 µm.

Article Snippet: The primers were designed using Vector NTI 9.1.0 (Thermo Fisher Scientific, Waltham, MA, USA) ( ). cDNA was synthesized from 1 µg RNA of Salmonella control culture or 3 µg RNA of Salmonella - Acanthamoeba co-culture using the M-MuLV Reverse Transcriptase (New England Biolabs, Ipswich, MA, USA).

Techniques: Infection, Confocal Laser Scanning Microscopy, Fluorescence